FastQCFastQC Report
Sun 19 Nov 2017
SRR957824_500K_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR957824_500K_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500000
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCTATCTCGTATGC6605813.2116TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA87050.0110.021719
AGAGCAC89250.0107.309698
AAGAGCA91750.0104.385737
GAAGAGC93600.0102.25596
TCGGAAG94150.0101.592273
GGAAGAG94300.0101.420525
ATCGGAA96100.099.5308152
GATCGGA96650.099.058711
CGGAAGA97000.098.5232854
CTTGAAA67800.027.68413460-64
TCTCGTA68900.027.59859540-44
CTCGTAT69150.027.58208340-44
GCTTGAA69150.027.53505555-59
TCGTATG69800.027.44896740-44
TGCTTGA69850.027.3415955-59
GTCTTCT70300.027.27421650-54
CTGCTTG70750.027.07521455-59
TATCTCG70200.027.00440835-39
ATCTCGT69850.026.97593940-44
GCCGTCT71350.026.89463245-49
Produced by FastQC (version 0.11.5)